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non-targeting shrna control (shctrl: shc016)  (Millipore)


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    Structured Review

    Millipore non-targeting shrna control (shctrl: shc016)
    (A) Western blot analysis to validate the knockdown efficiency of <t>shRNA</t> <t>against</t> <t>Fau</t> (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.
    Non Targeting Shrna Control (Shctrl: Shc016), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-targeting+shrna+control+%28shctrl%3A+shc016%29/bio_rxiv__2023__11__08__566330-329-12-20?v=Millipore
    Average 90 stars, based on 1 article reviews
    non-targeting shrna control (shctrl: shc016) - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Maternal sterol 27-hydroxylase is crucial for securing fetal development"

    Article Title: Maternal sterol 27-hydroxylase is crucial for securing fetal development

    Journal: bioRxiv

    doi: 10.1101/2023.11.08.566330

    (A) Western blot analysis to validate the knockdown efficiency of shRNA against Fau (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: (A) Western blot analysis to validate the knockdown efficiency of shRNA against Fau (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.

    Techniques Used: Western Blot, shRNA, Staining, Flow Cytometry, Expressing, In Vitro, Cell Culture, Transduction



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    non-targeting shrna control (shctrl: shc016)  (Millipore)
    90
    Millipore non-targeting shrna control (shctrl: shc016)
    (A) Western blot analysis to validate the knockdown efficiency of <t>shRNA</t> <t>against</t> <t>Fau</t> (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.
    Non Targeting Shrna Control (Shctrl: Shc016), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-targeting+shrna+control+%28shctrl%3A+shc016%29/bio_rxiv__2023__11__08__566330-329-12-20?v=Millipore
    Average 90 stars, based on 1 article reviews
    non-targeting shrna control (shctrl: shc016) - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blot analysis to validate the knockdown efficiency of shRNA against Fau (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.

    Journal: bioRxiv

    Article Title: Maternal sterol 27-hydroxylase is crucial for securing fetal development

    doi: 10.1101/2023.11.08.566330

    Figure Lengend Snippet: (A) Western blot analysis to validate the knockdown efficiency of shRNA against Fau (shFau). Non-targeting control shRNA (shCtrl) was used as a control. (B) Protein synthesis rate analysis using OP-Puro staining. A representative flow cytometry analysis of OP-Puro intensity in the LLC expressing shCtrl or shFau is shown. (C) Polysome profiling of LLC expressing shFau driven by the Tet-On inducible system. (D) A schema of the in vitro culture of fetal lung expressing shFau. (E) Representative photos of the fetal lungs cultured in the air-liquid interface system. The scale bar represents 1 mm. (F) The number of branching points in the fetal lungs transduced with lentiviruses. (G) Representative Western blot analysis of the fetal lungs transduced with lentiviruses. (H) The relative signal of Fau and Sftpc. The intensity of the band was normalized using Actb, and the fold changes in the shFau samples compared to the DMSO control is shown. * p < 0.05, ** p < 0.01.

    Article Snippet: Mission lentiviral shRNA plasmids targeting Fau (shFau: TRCN0000104130, TRCN0000104131, TRCN0000104132, and TRCN0000104133), non-targeting shRNA control (shCtrl: SHC016) were purchased from Sigma-Aldrich.

    Techniques: Western Blot, shRNA, Staining, Flow Cytometry, Expressing, In Vitro, Cell Culture, Transduction